Activation of mitogen-activated protein kinase associated with prostate cancer progression

D Gioeli, JW Mandell, GR Petroni, HF Frierson Jr… - Cancer research, 1999 - AACR
D Gioeli, JW Mandell, GR Petroni, HF Frierson Jr, MJ Weber
Cancer research, 1999AACR
Using an antibody specific for dually phosphorylated extracellular-regulated kinases 1 and
2, we have examined 82 primary and metastatic prostate tumor specimens for the presence
of activated mitogen-activated protein (MAP) kinase. Nonneoplastic prostate tissue showed
little or no staining with activated MAP kinase antiserum. In prostate tumors, the level of
activated MAP kinase increased with increasing Gleason score and tumor stage. In a
separate analysis, tumor samples from two patients showed no activation of MAP kinase …
Abstract
Using an antibody specific for dually phosphorylated extracellular-regulated kinases 1 and 2, we have examined 82 primary and metastatic prostate tumor specimens for the presence of activated mitogen-activated protein (MAP) kinase. Nonneoplastic prostate tissue showed little or no staining with activated MAP kinase antiserum. In prostate tumors, the level of activated MAP kinase increased with increasing Gleason score and tumor stage. In a separate analysis, tumor samples from two patients showed no activation of MAP kinase before androgen ablation therapy; however, following androgen ablation treatment, high levels of activated MAP kinase were detected in the recurrent tumors. Collectively, these data suggest an increase in the activation of the MAP kinase signal transduction pathway as prostate cancer progresses to a more advanced and androgen-independent disease.
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