Amine reactive dyes: an effective tool to discriminate live and dead cells in polychromatic flow cytometry
SP Perfetto, PK Chattopadhyay, L Lamoreaux… - Journal of …, 2006 - Elsevier
Membrane-damaged cells caused by either mechanical trauma or through normal biological
processes can produce artifacts in immunophenotyping analysis by flow cytometry. Dead
cells can nonspecifically bind monoclonal antibody conjugates, potentially leading to
erroneous conclusions, particularly when cell frequencies are low. To date, DNA
intercalating dyes (Ethidium monoazaide (EMA), Propidium Iodide, 7AAD, etc.) or Annexin V
have been commonly used to exclude dead cells; however, each suffer from technical …
processes can produce artifacts in immunophenotyping analysis by flow cytometry. Dead
cells can nonspecifically bind monoclonal antibody conjugates, potentially leading to
erroneous conclusions, particularly when cell frequencies are low. To date, DNA
intercalating dyes (Ethidium monoazaide (EMA), Propidium Iodide, 7AAD, etc.) or Annexin V
have been commonly used to exclude dead cells; however, each suffer from technical …