[HTML][HTML] Overexpression of catalase in cytosolic or mitochondrial compartment protects HepG2 cells against oxidative injury
J Bai, AM Rodriguez, JA Melendez… - Journal of Biological …, 1999 - ASBMB
HepG2 cells were transfected with vectors containing human catalase cDNA and catalase
cDNA with a mitochondrial leader sequence to allow comparison of the effectiveness of
catalase overexpressed in the cytosolic or mitochondrial compartments to protect against
oxidant-induced injury. Overexpression of catalase in cytosol and in mitochondria was
confirmed by Western blot, and activity measurement and stable cell lines were established.
The intracellular level of H 2 O 2 induced by exogenously added H 2 O 2 or antimycin A was …
cDNA with a mitochondrial leader sequence to allow comparison of the effectiveness of
catalase overexpressed in the cytosolic or mitochondrial compartments to protect against
oxidant-induced injury. Overexpression of catalase in cytosol and in mitochondria was
confirmed by Western blot, and activity measurement and stable cell lines were established.
The intracellular level of H 2 O 2 induced by exogenously added H 2 O 2 or antimycin A was …