[PDF][PDF] The WAVE2 complex regulates actin cytoskeletal reorganization and CRAC-mediated calcium entry during T cell activation
JC Nolz, TS Gomez, P Zhu, S Li, RB Medeiros… - Current biology, 2006 - cell.com
Current biology, 2006•cell.com
Background The engagement of the T cell receptor results in actin cytoskeletal
reorganization at the immune synapse (IS) and the triggering of biochemical signaling
cascades leading to gene regulation and, ultimately, cellular activation. Recent studies have
identified the WAVE family of proteins as critical mediators of Rac1-induced actin
reorganization in other cell types. However, whether these proteins participate in actin
reorganization at the IS or signaling pathways in T cells has not been investigated. Results …
reorganization at the immune synapse (IS) and the triggering of biochemical signaling
cascades leading to gene regulation and, ultimately, cellular activation. Recent studies have
identified the WAVE family of proteins as critical mediators of Rac1-induced actin
reorganization in other cell types. However, whether these proteins participate in actin
reorganization at the IS or signaling pathways in T cells has not been investigated. Results …
Background
The engagement of the T cell receptor results in actin cytoskeletal reorganization at the immune synapse (IS) and the triggering of biochemical signaling cascades leading to gene regulation and, ultimately, cellular activation. Recent studies have identified the WAVE family of proteins as critical mediators of Rac1-induced actin reorganization in other cell types. However, whether these proteins participate in actin reorganization at the IS or signaling pathways in T cells has not been investigated.
Results
By using a combination of biochemical, genetic, and cell biology approaches, we provide evidence that WAVE2 is recruited to the IS, is biochemically modified, and is required for actin reorganization and β-integrin-mediated adhesion after TCR crosslinking. Moreover, we show that WAVE2 regulates calcium entry at a point distal to PLCγ1 activation and IP3-mediated store release.
Conclusions
These data reveal a role for WAVE2 in regulating multiple pathways leading to T cell activation. In particular, this work shows that WAVE2 is a key component of the actin regulatory machinery in T cells and that it also participates in linking intracellular calcium store depletion to calcium release-activated calcium (CRAC) channel activation.
cell.com