Putative glycophorin-binding protein is secreted from schizonts of Plasmodium falciparum
AE Bianco, JG Culvenor, RL Coppel… - Molecular and …, 1987 - Elsevier
A cDNA clone expressing an antigen of Plasmodium falciparum, selected by screening an
expression library cloned in Escherichia coli, encodes a portion of the protein identified as a
glycophorin-binding protein [Kochan et al.(1986) Cell 44, 689–696]. Human antibodies
affinity-purified on extracts from this clone were used to characterize the antigen by
immunoblotting. This protein was present in all isolates tested, restricted to mature
trophozoites and schizonts. It was abundant in culture supernatants at the time of merozoite …
expression library cloned in Escherichia coli, encodes a portion of the protein identified as a
glycophorin-binding protein [Kochan et al.(1986) Cell 44, 689–696]. Human antibodies
affinity-purified on extracts from this clone were used to characterize the antigen by
immunoblotting. This protein was present in all isolates tested, restricted to mature
trophozoites and schizonts. It was abundant in culture supernatants at the time of merozoite …