Mapping of the IgE and IgG4 sequential epitopes of milk allergens with a peptide microarray–based immunoassay
I Cerecedo, J Zamora, WG Shreffler, J Lin… - Journal of Allergy and …, 2008 - Elsevier
BACKGROUND: Peptide microarray analysis is a novel method that can provide useful
information on the nature of specific allergies. OBJECTIVE: We sought to determine the
specificity and diversity of IgE and IgG4 antibodies binding to sequential epitopes of αs1-,
αs2-, β-, and κ-caseins and β-lactoglobulin by using a peptide microarray–based
immunoassay. METHODS: A microarray immunoassay was performed with sera from 31
children with IgE-mediated milk allergy (16 with positive oral milk challenge results [ie, the …
information on the nature of specific allergies. OBJECTIVE: We sought to determine the
specificity and diversity of IgE and IgG4 antibodies binding to sequential epitopes of αs1-,
αs2-, β-, and κ-caseins and β-lactoglobulin by using a peptide microarray–based
immunoassay. METHODS: A microarray immunoassay was performed with sera from 31
children with IgE-mediated milk allergy (16 with positive oral milk challenge results [ie, the …