Microarray immunoassay: association of clinical history, in vitro IgE function, and heterogeneity of allergenic peanut epitopes

WG Shreffler, K Beyer, THT Chu, AW Burks… - Journal of allergy and …, 2004 - Elsevier
BACKGROUND: IgE epitope mapping of food allergens is a prerequisite for engineering
hypoallergenic immunotherapeutic agents and might reveal basic information regarding a
patient's immune response. Mapping of large numbers of epitopes by using individual
patient sera has been impractical with current techniques. OBJECTIVE: We sought to
develop a peptide microarray-based immunoassay to map peanut epitopes by using
microliter quantities of serum. METHODS: A set of 213 overlapping 20-residue peptides was …