Specificity of IgE antibodies to sequential epitopes of hen's egg ovomucoid as a marker for persistence of egg allergy
KM Järvinen, K Beyer, L Vila, L Bardina, M Mishoe… - Allergy, 2007 - Wiley Online Library
KM Järvinen, K Beyer, L Vila, L Bardina, M Mishoe, HA Sampson
Allergy, 2007•Wiley Online LibraryBackground: Approximately two‐thirds of egg‐allergic infants become tolerant within the first
5 years of life. Objective: We sought (1) to compare the recognition of sequential (linear) and
conformational binding sites of ovomucoid, ovalbumin and ovotransferrin, by IgE antibodies
of children with persistent and transient egg allergy,(2) to identify immunodominant IgE‐and
IgG‐binding epitopes of ovomucoid, and (3) to compare epitope‐specificity of IgE antibodies
between patients with differing natural histories of egg allergy. Methods: Using immunodot …
5 years of life. Objective: We sought (1) to compare the recognition of sequential (linear) and
conformational binding sites of ovomucoid, ovalbumin and ovotransferrin, by IgE antibodies
of children with persistent and transient egg allergy,(2) to identify immunodominant IgE‐and
IgG‐binding epitopes of ovomucoid, and (3) to compare epitope‐specificity of IgE antibodies
between patients with differing natural histories of egg allergy. Methods: Using immunodot …
Background: Approximately two‐thirds of egg‐allergic infants become tolerant within the first 5 years of life.
Objective: We sought (1) to compare the recognition of sequential (linear) and conformational binding sites of ovomucoid, ovalbumin and ovotransferrin, by IgE antibodies of children with persistent and transient egg allergy, (2) to identify immunodominant IgE‐and IgG‐binding epitopes of ovomucoid, and (3) to compare epitope‐specificity of IgE antibodies between patients with differing natural histories of egg allergy.
Methods: Using immunodot‐blots or ImmunoCAPs, IgE‐antibodies against conformational (native) and sequential (reduced and alkylated) egg proteins were determined at the time of clinical reactivity in patients who retained their allergy and in those who developed clinical tolerance. IgE‐ and IgG‐binding epitopes were mapped for ovomucoid using overlapping decapeptides on SPOTs membranes. Recognition of the major IgE‐binding epitopes were compared between patients with differing natural histories of egg allergy.
Results: The patients with long‐lasting egg allergy had a higher concentrations of IgE antibodies against sequential and native ovomucoid and ovalbumin than the children who subsequently gained tolerance (P < 0.01). Four major IgE‐binding epitopes were identified in ovomucoid at amino acid 1–10, 9–20, 47–56, and 113–124. IgE antibodies of all seven patients with persistent egg allergy recognized these epitopes whereas none of the 11 children who outgrew their egg allergy did so.
Conclusions: Patients with persistent egg allergy develop IgE antibodies against more sequential and conformational epitopes of ovomucoid and ovalbumin. The presence of serum IgE antibodies to specific sequential epitopes of ovomucoid may be used as a screening instrument for persistent egg allergy.
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