Isolation and native characterization of cysteine-rich collagens from bovine placental tissues and uterus and their relationship to types IV and V collagens
MZ Abedin, S Ayad, JB Weiss - Bioscience reports, 1982 - portlandpress.com
MZ Abedin, S Ayad, JB Weiss
Bioscience reports, 1982•portlandpress.comA simplified procedure for the fractionation and purification of different collagen types from
various tissues is described which is particularly efficient in separating type-V from type-IV
collagen, and highmol.-wt.(HMW) aggregates from 7 S collagen. Uterus and maternal villi
contain 2 forms of type-V collagen−{α1 (V)} 2α2 (V) and {α1 (V) 2α2 (V) α3 (V)}–which have
been separated on DEAE-cellulose. Uterus however appears to be the richest source of
both HMW aggregates and the {α1 (V) 2α2 (V) α3 (V)} collagen, and a probable relationship …
various tissues is described which is particularly efficient in separating type-V from type-IV
collagen, and highmol.-wt.(HMW) aggregates from 7 S collagen. Uterus and maternal villi
contain 2 forms of type-V collagen−{α1 (V)} 2α2 (V) and {α1 (V) 2α2 (V) α3 (V)}–which have
been separated on DEAE-cellulose. Uterus however appears to be the richest source of
both HMW aggregates and the {α1 (V) 2α2 (V) α3 (V)} collagen, and a probable relationship …
A simplified procedure for the fractionation and purification of different collagen types from various tissues is described which is particularly efficient in separating type-V from type-IV collagen, and highmol.-wt. (HMW) aggregates from 7 S collagen. Uterus and maternal villi contain 2 forms of type-V collagen −{α1(V)}2α2(V) and {α1(V)2α2(V)α3(V)}–which have been separated on DEAE-cellulose. Uterus however appears to be the richest source of both HMW aggregates and the {α1(V)2α2(V)α3(V)} collagen, and a probable relationship between these collagens is discussed.
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