Metabolic Characterization of the R6/2 Transgenic Mouse Model of Huntington's Disease by High-Resolution MAS 1H NMR Spectroscopy
TM Tsang, B Woodman, GA Mcloughlin… - Journal of proteome …, 2006 - ACS Publications
Journal of proteome research, 2006•ACS Publications
The metabolic consequences of Huntington's disease in the R6/2 mouse model were
investigated using NMR spectroscopy and pattern recognition to characterize selected brain
regions, muscle, blood, and urine. Global increases in relative brain concentrations of
osmolytes, creatine, glutamine, and lactate, and decreases in acetate and N-acetylaspartate
were found together with striatal-specific lower concentrations of GABA and choline. Clear
differentiation of R6/2 and wild-type mice was also obtained for urine and blood metabolite …
investigated using NMR spectroscopy and pattern recognition to characterize selected brain
regions, muscle, blood, and urine. Global increases in relative brain concentrations of
osmolytes, creatine, glutamine, and lactate, and decreases in acetate and N-acetylaspartate
were found together with striatal-specific lower concentrations of GABA and choline. Clear
differentiation of R6/2 and wild-type mice was also obtained for urine and blood metabolite …
The metabolic consequences of Huntington's disease in the R6/2 mouse model were investigated using NMR spectroscopy and pattern recognition to characterize selected brain regions, muscle, blood, and urine. Global increases in relative brain concentrations of osmolytes, creatine, glutamine, and lactate, and decreases in acetate and N-acetylaspartate were found together with striatal-specific lower concentrations of GABA and choline. Clear differentiation of R6/2 and wild-type mice was also obtained for urine and blood metabolite profiles that may have applicability for monitoring HD in human populations.
Keywords: Huntington's disease • metabolic profiling • NMR spectroscopy • pattern recognition • R6/2 mice
ACS Publications