The death effector domain-containing DEDD forms a complex with Akt and Hsp90, and supports their stability

N Kurabe, M Mori, J Kurokawa, K Taniguchi… - Biochemical and …, 2010 - Elsevier
N Kurabe, M Mori, J Kurokawa, K Taniguchi, H Aoyama, K Atsuda, A Nishijima, N Odawara…
Biochemical and biophysical research communications, 2010Elsevier
Insulin secretion and glucose transport are the major mechanisms to balance glucose
homeostasis. Recently, we found that the death effector domain-containing DEDD inhibits
cyclin-dependent kinase-1 (Cdk1) function, thereby preventing Cdk1-dependent inhibitory
phosphorylation of S6 kinase-1 (S6K1), downstream of phosphatidylinositol 3-kinase (PI3K),
which overall results in maintenance of S6K1 activity. Here we newly show that DEDD forms
a complex with Akt and heat-shock protein 90 (Hsp90), and supports the stability of both …
Insulin secretion and glucose transport are the major mechanisms to balance glucose homeostasis. Recently, we found that the death effector domain-containing DEDD inhibits cyclin-dependent kinase-1 (Cdk1) function, thereby preventing Cdk1-dependent inhibitory phosphorylation of S6 kinase-1 (S6K1), downstream of phosphatidylinositol 3-kinase (PI3K), which overall results in maintenance of S6K1 activity. Here we newly show that DEDD forms a complex with Akt and heat-shock protein 90 (Hsp90), and supports the stability of both proteins. Hence, in DEDD−/− mice, Akt protein levels are diminished in skeletal muscles and adipose tissues, which interferes with the translocation of glucose-transporter 4 (GLUT4) upon insulin stimulation, leading to inefficient incorporation of glucose in these organs. Interestingly, as for the activation of S6K1, suppression of Cdk1 is involved in the stabilization of Akt protein by DEDD, since diminishment of Cdk1 in DEDD−/− cells via siRNA expression or treatment with a Cdk1-inhibitor, increases both Akt and Hsp90 protein levels. Such multifaceted involvement of DEDD in glucose homeostasis by supporting both insulin secretion (via maintenance of S6K1 activity) and glucose uptake (via stabilizing Akt protein), may suggest an association of DEDD-deficiency with the pathogenesis of type 2 diabetes mellitus.
Elsevier