The orphan receptors COUP-TF and HNF-4 serve as accessory factors required for induction of phosphoenolpyruvate carboxykinase gene transcription by …
RK Hall, FM Sladek… - Proceedings of the …, 1995 - National Acad Sciences
RK Hall, FM Sladek, DK Granner
Proceedings of the National Academy of Sciences, 1995•National Acad SciencesGlucocorticoids stimulate hepatic phosphoenolpyruvate carboxykinase (PEPCK; EC 4.1.
1.32) gene expression, thereby increasing the rate of gluconeogenesis. The effect of
glucocorticoids on PEPCK gene expression is mediated by a set of promoter elements
collectively referred to as the glucocorticoid response unit. The response unit spans a 100-
bp segment and includes two glucocorticoid receptor binding sites (GR1 and GR2) and two
accessory factor binding sites (AF1 and AF2), all of which are required for a maximal …
1.32) gene expression, thereby increasing the rate of gluconeogenesis. The effect of
glucocorticoids on PEPCK gene expression is mediated by a set of promoter elements
collectively referred to as the glucocorticoid response unit. The response unit spans a 100-
bp segment and includes two glucocorticoid receptor binding sites (GR1 and GR2) and two
accessory factor binding sites (AF1 and AF2), all of which are required for a maximal …
Glucocorticoids stimulate hepatic phosphoenolpyruvate carboxykinase (PEPCK; EC 4.1.1.32) gene expression, thereby increasing the rate of gluconeogenesis. The effect of glucocorticoids on PEPCK gene expression is mediated by a set of promoter elements collectively referred to as the glucocorticoid response unit. The response unit spans a 100-bp segment and includes two glucocorticoid receptor binding sites (GR1 and GR2) and two accessory factor binding sites (AF1 and AF2), all of which are required for a maximal glucocorticoid response. The AF1 element also serves as a retinoic acid response element and may be involved in developmental and tissue-specific expression of the gene. In this study we report that COUP-TF and HNF-4, two orphan members of the nuclear receptor superfamily, bind to the AF1 element and function as accessory factors for the glucocorticoid response of the PEPCK gene.
National Acad Sciences