Rescue of influenza A virus from recombinant DNA
E Fodor, L Devenish, OG Engelhardt, P Palese… - Journal of …, 1999 - Am Soc Microbiol
E Fodor, L Devenish, OG Engelhardt, P Palese, GG Brownlee, A García-Sastre
Journal of virology, 1999•Am Soc MicrobiolWe have rescued influenza A virus by transfection of 12 plasmids into Vero cells. The eight
individual negative-sense genomic viral RNAs were transcribed from plasmids containing
human RNA polymerase I promoter and hepatitis delta virus ribozyme sequences. The three
influenza virus polymerase proteins and the nucleoprotein were expressed from protein
expression plasmids. This plasmid-based reverse genetics technique facilitates the
generation of recombinant influenza viruses containing specific mutations in their genes.
individual negative-sense genomic viral RNAs were transcribed from plasmids containing
human RNA polymerase I promoter and hepatitis delta virus ribozyme sequences. The three
influenza virus polymerase proteins and the nucleoprotein were expressed from protein
expression plasmids. This plasmid-based reverse genetics technique facilitates the
generation of recombinant influenza viruses containing specific mutations in their genes.
Abstract
We have rescued influenza A virus by transfection of 12 plasmids into Vero cells. The eight individual negative-sense genomic viral RNAs were transcribed from plasmids containing human RNA polymerase I promoter and hepatitis delta virus ribozyme sequences. The three influenza virus polymerase proteins and the nucleoprotein were expressed from protein expression plasmids. This plasmid-based reverse genetics technique facilitates the generation of recombinant influenza viruses containing specific mutations in their genes.
American Society for Microbiology