[HTML][HTML] Classes of thiols that influence the activity of the skeletal muscle calcium release channel
J Sun, L Xu, JP Eu, JS Stamler, G Meissner - Journal of Biological …, 2001 - ASBMB
The skeletal muscle Ca 2+ release channel/ryanodine receptor (RyR1) is a prototypic redox-
responsive ion channel. Nearly half of the 101 cysteines per RyR1 subunit are kept in a
reduced (free thiol) state under conditions comparable with resting muscle. Here we
assessed the effects of physiological determinants of cellular redox state (oxygen tension,
reduced (GSH) or oxidized (GSSG) glutathione, and NO/O⨪ 2 (released by 3-
morpholinosydnonimine)) on RyR1 redox state and activity. Oxidation of∼ 10 RyR1 thiols …
responsive ion channel. Nearly half of the 101 cysteines per RyR1 subunit are kept in a
reduced (free thiol) state under conditions comparable with resting muscle. Here we
assessed the effects of physiological determinants of cellular redox state (oxygen tension,
reduced (GSH) or oxidized (GSSG) glutathione, and NO/O⨪ 2 (released by 3-
morpholinosydnonimine)) on RyR1 redox state and activity. Oxidation of∼ 10 RyR1 thiols …