Cassette vectors directing expression of T cell receptor genes in transgenic mice
V Kouskoff, K Signorelli, C Benoist, D Mathis - Journal of immunological …, 1995 - Elsevier
We describe a pair of cassette vectors that can be used to express rearranged T cell
receptor genes in transgenic mice. Short DNA fragments containing rearranged Vα and Vβ
segments are readily amplified from T cells and introduced between artificial cloning sites.
Transgene-derived mRNAs are transcribed under the control of the natural TCRα and-β
promoter/enhancer elements. Using this vector, we have obtained transgenic mouse lines
which display transgene-encoded TCR α and β chains on a majority of T cells.
receptor genes in transgenic mice. Short DNA fragments containing rearranged Vα and Vβ
segments are readily amplified from T cells and introduced between artificial cloning sites.
Transgene-derived mRNAs are transcribed under the control of the natural TCRα and-β
promoter/enhancer elements. Using this vector, we have obtained transgenic mouse lines
which display transgene-encoded TCR α and β chains on a majority of T cells.