[HTML][HTML] Mode of growth hormone action in osteoblasts
DJ DiGirolamo, A Mukherjee, K Fulzele, Y Gan… - Journal of Biological …, 2007 - ASBMB
Growth hormone (GH) affects bone size and mass in part through stimulating insulin-like
growth factor type 1 (IGF-1) production in liver and bone. Whether GH acts independent of
IGF-1 in bone remains unclear. To define the mode of GH action in bone, we have used a
Cre/loxP system in which the type 1 IGF-1 receptor (Igf1r) has been disrupted specifically in
osteoblasts in vitro and in vivo. Calvarial osteoblasts from mice homozygous for the floxed
IGF-1R allele (IGF-1R flox/flox) were infected with adenoviral vectors expressing Cre …
growth factor type 1 (IGF-1) production in liver and bone. Whether GH acts independent of
IGF-1 in bone remains unclear. To define the mode of GH action in bone, we have used a
Cre/loxP system in which the type 1 IGF-1 receptor (Igf1r) has been disrupted specifically in
osteoblasts in vitro and in vivo. Calvarial osteoblasts from mice homozygous for the floxed
IGF-1R allele (IGF-1R flox/flox) were infected with adenoviral vectors expressing Cre …