Inducible nitric oxide synthase: correlation with extracapsular spread and enhancement of tumor cell invasion in head and neck squamous cell carcinoma
PA Brennan, S Dennis, D Poller… - Head & Neck …, 2008 - Wiley Online Library
Head & Neck: Journal for the Sciences and Specialties of the Head …, 2008•Wiley Online Library
Background Extracapsular nodal spread is a major prognostic indicator in head and neck
cancer. Nitric oxide (NO), primarily produced by the enzyme inducible NO synthase (iNOS),
has a large number of actions in cancer biology, but no studies have investigated its
possible role in extracapsular spread or tumor invasion. Methods Immunochemistry was
used to study iNOS expression in 48 patients with either extracapsular or encapsulated
metastasis. In vitro invasion assays were performed using H357 (an oral squamous cell …
cancer. Nitric oxide (NO), primarily produced by the enzyme inducible NO synthase (iNOS),
has a large number of actions in cancer biology, but no studies have investigated its
possible role in extracapsular spread or tumor invasion. Methods Immunochemistry was
used to study iNOS expression in 48 patients with either extracapsular or encapsulated
metastasis. In vitro invasion assays were performed using H357 (an oral squamous cell …
Background
Extracapsular nodal spread is a major prognostic indicator in head and neck cancer. Nitric oxide (NO), primarily produced by the enzyme inducible NO synthase (iNOS), has a large number of actions in cancer biology, but no studies have investigated its possible role in extracapsular spread or tumor invasion.
Methods
Immunochemistry was used to study iNOS expression in 48 patients with either extracapsular or encapsulated metastasis. In vitro invasion assays were performed using H357 (an oral squamous cell carinoma cell line) using the iNOS inhibitor drug, 1400 W.
Results
iNOS expression was significantly associated with extracapsular spread, with 22/27 cases showing positive iNOS expression compared with 8/21 cases in the encapsulated group (p = .01). Invasion of H357 cells was inhibited by 1400 W at concentrations of 100 μM and 1 mM (p = .002, p = .003).
Conclusion
iNOS protein seems to be associated with extracapsular spread and invasion in head and neck cancer. Further studies are required to understand this role more fully. © 2007 Wiley Periodicals, Inc. Head Neck, 2008
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