Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector.
H Shizuya, B Birren, UJ Kim… - Proceedings of the …, 1992 - National Acad Sciences
H Shizuya, B Birren, UJ Kim, V Mancino, T Slepak, Y Tachiiri, M Simon
Proceedings of the National Academy of Sciences, 1992•National Acad SciencesA bacterial cloning system for mapping and analysis of complex genomes has been
developed. The BAC system (for bacterial artificial chromosome) is based on Escherichia
coli and its single-copy plasmid F factor. It is capable of maintaining human genomic DNA
fragments of greater than 300 kilobase pairs. Individual clones of human DNA appear to be
maintained with a high degree of structural stability in the host, even after 100 generations of
serial growth. Because of high cloning efficiency, easy manipulation of the cloned DNA, and …
developed. The BAC system (for bacterial artificial chromosome) is based on Escherichia
coli and its single-copy plasmid F factor. It is capable of maintaining human genomic DNA
fragments of greater than 300 kilobase pairs. Individual clones of human DNA appear to be
maintained with a high degree of structural stability in the host, even after 100 generations of
serial growth. Because of high cloning efficiency, easy manipulation of the cloned DNA, and …
A bacterial cloning system for mapping and analysis of complex genomes has been developed. The BAC system (for bacterial artificial chromosome) is based on Escherichia coli and its single-copy plasmid F factor. It is capable of maintaining human genomic DNA fragments of greater than 300 kilobase pairs. Individual clones of human DNA appear to be maintained with a high degree of structural stability in the host, even after 100 generations of serial growth. Because of high cloning efficiency, easy manipulation of the cloned DNA, and stable maintenance of inserted DNA, the BAC system may facilitate construction of DNA libraries of complex genomes with fuller representation and subsequent rapid analysis of complex genomic structure.
National Acad Sciences