Quantitative measurement of anti-ErbB-2 antibody by flow cytometry and ELISA
MP Piechocki, SA Pilon, WZ Wei - Journal of immunological methods, 2002 - Elsevier
To establish standard methods for measuring anti-ErbB-2 antibody, a flow cytometric and an
enzyme-linked immunosorbent assay (ELISA) were developed and compared. In the flow
cytometric assay, the antibody was measured by binding to human breast cancer cell line
SKBR3 and the result expressed as mean channel fluorescence (MCF). In ELISA, the
antibody was measured by binding to a recombinant, secreted human ErbB-2 containing the
N-terminal 505 amino acids of ErbB-2 fused to myc and His tags (secE2/myc/His or secE2) …
enzyme-linked immunosorbent assay (ELISA) were developed and compared. In the flow
cytometric assay, the antibody was measured by binding to human breast cancer cell line
SKBR3 and the result expressed as mean channel fluorescence (MCF). In ELISA, the
antibody was measured by binding to a recombinant, secreted human ErbB-2 containing the
N-terminal 505 amino acids of ErbB-2 fused to myc and His tags (secE2/myc/His or secE2) …