Identification of semaphorin E gene expression in metastatic human lung adenocarcinoma cells by mRNA differential display

M Martín‐Satué, J Blanco - Journal of surgical oncology, 1999 - Wiley Online Library
M Martín‐Satué, J Blanco
Journal of surgical oncology, 1999Wiley Online Library
Synopsis Human lung adenocarcinoma cell lines HAL‐8Luc and HAL‐24Luc differ in their
metastatic potential. HAL‐8Luc cells metastasize to lungs when injected either intravenously
or intramuscularly. in mice while HAL‐24Luc cells do not. The differential display method is
used to identify genes differentially expressed between the two cell lines and the findings
are extensively discussed. Background Lung cancer is the leading form of cancer in most
countries, and metastasis is the main cause of death in oncological patients. The metastatic …
Synopsis
Human lung adenocarcinoma cell lines HAL‐8Luc and HAL‐24Luc differ in their metastatic potential. HAL‐8Luc cells metastasize to lungs when injected either intravenously or intramuscularly. in mice while HAL‐24Luc cells do not. The differential display method is used to identify genes differentially expressed between the two cell lines and the findings are extensively discussed.
Background
Lung cancer is the leading form of cancer in most countries, and metastasis is the main cause of death in oncological patients. The metastatic phenotype of tumor cells is the result of genetic events altering the RNA and protein expression of normal cells. Our objective was to identify genes expressed differentially between metastatic and nonmetastatic human lung adenocarcinoma cells that might be used as a prognostic factor.
Methods
The differential display technique was used to compare the RNA expression patterns distinguishing metastatic (HAL‐8Luc) and nonmetastatic (HAL‐24Luc) human lung adenocarcinoma cells, two genetically close cell lines.
Results
Differential expression of three cDNAs was confirmed by Northern blot analysis. Two sequences corresponding to a putative splicing factor and a proliferation‐related factor cDNAs were underexpressed in the metastatic cells relative to the nonmetastatic ones. Interestingly, we found that human semaphorin E mRNA was several fold overexpressed in the metastatic cells. This recently identified gene encodes a protein whose expression has been related to several cell survival mechanisms as well as to immunosuppression.
Conclusion
Our results point to the relevance of semaphorin E in metastatic spread of human lung adenocarcinoma cells. J. Surg. Oncol. 1999;72:18–23. © 1999 Wiley‐Liss, Inc.
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