[HTML][HTML] Site-directed mutagenesis in the B-neuropilin-2 domain selectively enhances its affinity to VEGF165, but not to semaphorin 3F

E Geretti, A Shimizu, P Kurschat… - Journal of Biological …, 2007 - ASBMB
Neuropilins (NRPs) are 130-kDa receptors that bind and respond to the class 3 semaphorin
family of axon guidance molecules (SEMAs) and to members of the vascular endothelial
growth factor (VEGF) family of angiogenic factors. Two NRPs have been reported so far,
NRP1 and NRP2. Unlike NRP1, little is known about NRP2 interactions with its ligands,
VEGF 165 and SEMA3F. Cell binding studies reveal that VEGF 165 and SEMA3F bind
NRP2 with similar affinities, 5.2 and 3.9 nm, respectively, and are competitive NRP2 ligands …