[HTML][HTML] Co-translational degradation of apolipoprotein B100 by the proteasome is prevented by microsomal triglyceride transfer protein: synchronized translation …
F Benoist, T Grand-Perret - Journal of Biological Chemistry, 1997 - ASBMB
We studied the effect of inhibition of microsomal triglyceride transfer protein (MTP) on
apolipoprotein (apo) B100 translation and secretion using HepG2 cells. The MTP-mediated
lipid transfer activity was reduced using a specific MTP inhibitor. ApoB100 translation was
synchronized by treatment with puromycin prior tol-[35 S] methionine pulse-chase labeling.
During the first 4 min of chase, synthesis of apoB polypeptides the size of 100–200 kDa was
insensitive to the inhibitor, suggesting that inhibition of MTP did not affect the initiation of …
apolipoprotein (apo) B100 translation and secretion using HepG2 cells. The MTP-mediated
lipid transfer activity was reduced using a specific MTP inhibitor. ApoB100 translation was
synchronized by treatment with puromycin prior tol-[35 S] methionine pulse-chase labeling.
During the first 4 min of chase, synthesis of apoB polypeptides the size of 100–200 kDa was
insensitive to the inhibitor, suggesting that inhibition of MTP did not affect the initiation of …