Rapid modification of bacterial artificial chromosomes by ET-recombination

JPP Muyrers, Y Zhang, G Testa… - Nucleic acids …, 1999 - academic.oup.com
JPP Muyrers, Y Zhang, G Testa, AF Stewart
Nucleic acids research, 1999academic.oup.com
We present a method to modify bacterial artificial chromosomes (BACs) resident in their host
strain. The method is based on homologous recombination by ET-cloning. We have
successfully modified BACs at two distinct loci by recombination with a PCR product
containing homology arms of 50 nt. The procedure we describe here is rapid, was found to
work with high efficiency and should be applicable to any BAC modification desired.
Abstract
We present a method to modify bacterial artificial chromosomes (BACs) resident in their host strain. The method is based on homologous recombination by ET-cloning. We have successfully modified BACs at two distinct loci by recombination with a PCR product containing homology arms of 50 nt. The procedure we describe here is rapid, was found to work with high efficiency and should be applicable to any BAC modification desired.
Oxford University Press