Stable and sensitive method for the simultaneous determination of N5-methyltetrahydrofolate, leucovorin, methotrexate and 7-hydroxymethotrexate in biological fluids
O Van Tellingen, HR Van Der Woude… - … of Chromatography B …, 1989 - Elsevier
A high-performance liquid chromatographic method for the determination of N 5-
methyltetrahydrofolic acid, leucovorin, methotrexate and 7-hydroxymethotrexate in plasma
and liquor samples is presented. Gradient elution is used to increase the sensitivity. Four
sample preparation methods were compared with respect to the stability of the injectable
sample. Samples can be pretreated with a simple deproteinization method. For enhanced
selectivity a solid-phase extraction procedure is described.
methyltetrahydrofolic acid, leucovorin, methotrexate and 7-hydroxymethotrexate in plasma
and liquor samples is presented. Gradient elution is used to increase the sensitivity. Four
sample preparation methods were compared with respect to the stability of the injectable
sample. Samples can be pretreated with a simple deproteinization method. For enhanced
selectivity a solid-phase extraction procedure is described.