[HTML][HTML] HIF-1α expression regulates the bactericidal capacity of phagocytes

C Peyssonnaux, V Datta, T Cramer… - The Journal of …, 2005 - Am Soc Clin Investig
C Peyssonnaux, V Datta, T Cramer, A Doedens, EA Theodorakis, RL Gallo, N Hurtado-Ziola…
The Journal of clinical investigation, 2005Am Soc Clin Investig
Hypoxia is a characteristic feature of the tissue microenvironment during bacterial infection.
Here we report on our use of conditional gene targeting to examine the contribution of
hypoxia-inducible factor 1, α subunit (HIF-1α) to myeloid cell innate immune function. HIF-1α
was induced by bacterial infection, even under normoxia, and regulated the production of
key immune effector molecules, including granule proteases, antimicrobial peptides, nitric
oxide, and TNF-α. Mice lacking HIF-1α in their myeloid cell lineage showed decreased …
Hypoxia is a characteristic feature of the tissue microenvironment during bacterial infection. Here we report on our use of conditional gene targeting to examine the contribution of hypoxia-inducible factor 1, α subunit (HIF-1α) to myeloid cell innate immune function. HIF-1α was induced by bacterial infection, even under normoxia, and regulated the production of key immune effector molecules, including granule proteases, antimicrobial peptides, nitric oxide, and TNF-α. Mice lacking HIF-1α in their myeloid cell lineage showed decreased bactericidal activity and failed to restrict systemic spread of infection from an initial tissue focus. Conversely, activation of the HIF-1α pathway through deletion of von Hippel–Lindau tumor-suppressor protein or pharmacologic inducers supported myeloid cell production of defense factors and improved bactericidal capacity. HIF-1α control of myeloid cell activity in infected tissues could represent a novel therapeutic target for enhancing host defense.
The Journal of Clinical Investigation