Differential staining of cartilage and bone in whole mouse fetuses by alcian blue and alizarin red S

MJ McLeod - Teratology, 1980 - Wiley Online Library
MJ McLeod
Teratology, 1980Wiley Online Library
The procedure described by Inouye ('76) for the staining of full‐term mouse fetal skeletons
has been adapted for use with mouse embryos and fetuses of days 14–18 of gestation. The
main adaptations for younger specimens involve a longer time in acetone, in lieu of
skinning, and omission of the aqueous KOH step. These adaptations require more time but
result in consistently good staining of intact specimens.
Abstract
The procedure described by Inouye ('76) for the staining of full‐term mouse fetal skeletons has been adapted for use with mouse embryos and fetuses of days 14–18 of gestation. The main adaptations for younger specimens involve a longer time in acetone, in lieu of skinning, and omission of the aqueous KOH step. These adaptations require more time but result in consistently good staining of intact specimens.
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