Accumulation of T cells with potent regulatory properties and restricted Vβ7-TCR rearrangements in tolerated allografts
JM Heslan, G Beriou, JB Le Luduec… - …, 2005 - journals.lww.com
Transplantation, 2005•journals.lww.com
Background. We have previously demonstrated that a short-course treatment with LF15-
0195, a 15-deoxyspergualin analogue, induces donor-specific tolerance of cardiac allografts
in rats and expansion of splenic CD4+ CD25+ regulatory T cells. Methods. To further
characterize long-term tolerance in this model, we have analyzed the phenotype, regulatory
properties and TCR-Vβ usage of the T cells infiltrating the tolerated allografts. Results. We
demonstrate that the tolerated allografts express high levels of FoxP3 transcripts and contain …
0195, a 15-deoxyspergualin analogue, induces donor-specific tolerance of cardiac allografts
in rats and expansion of splenic CD4+ CD25+ regulatory T cells. Methods. To further
characterize long-term tolerance in this model, we have analyzed the phenotype, regulatory
properties and TCR-Vβ usage of the T cells infiltrating the tolerated allografts. Results. We
demonstrate that the tolerated allografts express high levels of FoxP3 transcripts and contain …
Abstract
Background.
We have previously demonstrated that a short-course treatment with LF15-0195, a 15-deoxyspergualin analogue, induces donor-specific tolerance of cardiac allografts in rats and expansion of splenic CD4+ CD25+ regulatory T cells.
Methods.
To further characterize long-term tolerance in this model, we have analyzed the phenotype, regulatory properties and TCR-Vβ usage of the T cells infiltrating the tolerated allografts.
Results.
We demonstrate that the tolerated allografts express high levels of FoxP3 transcripts and contain a large number of CD4+ T cells, half of which express CD25. Moreover, T cells from these tolerated allografts are very powerful at transferring tolerance to a subsequent allograft recipient, demonstrating the presence of potent regulatory T cells at the site of the graft. Interestingly, the T cells infiltrating the tolerated allografts systematically display restricted Vβ7 TCR rearrangements.
Conclusion.
These results demonstrate in this model of tolerance, a specific accumulation of T cells with potent regulatory properties and exhibiting restricted Vβ7-TCR rearrangements at the graft site.
Lippincott Williams & Wilkins