Liposome mediated depletion of macrophages: mechanism of action, preparation of liposomes and applications
N Van Rooijen, A Sanders - Journal of immunological methods, 1994 - Elsevier
Selective depletion of macrophages from tissues in vivo can be used to investigate whether
these cells are playing a role in defined biological processes. This question is particularly
relevant to various host defense mechanisms. We have developed a macrophage
'suicide'technique, using the liposome mediated intracellular delivery of dichloromethylene-
bisphosphonate (Cl 2 MBP or clodronate). The method is specific with respect to phagocytic
cells of the mononuclear phagocyte system (MPS) for the following reasons:(1) The natural …
these cells are playing a role in defined biological processes. This question is particularly
relevant to various host defense mechanisms. We have developed a macrophage
'suicide'technique, using the liposome mediated intracellular delivery of dichloromethylene-
bisphosphonate (Cl 2 MBP or clodronate). The method is specific with respect to phagocytic
cells of the mononuclear phagocyte system (MPS) for the following reasons:(1) The natural …