[HTML][HTML] SLAM family receptors distinguish hematopoietic stem and progenitor cells and reveal endothelial niches for stem cells

MJ Kiel, ÖH Yilmaz, T Iwashita, OH Yilmaz, C Terhorst… - cell, 2005 - cell.com
MJ Kiel, ÖH Yilmaz, T Iwashita, OH Yilmaz, C Terhorst, SJ Morrison
cell, 2005cell.com
To improve our ability to identify hematopoietic stem cells (HSCs) and their localization in
vivo, we compared the gene expression profiles of highly purified HSCs and non-self-
renewing multipotent hematopoietic progenitors (MPPs). Cell surface receptors of the SLAM
family, including CD150, CD244, and CD48, were differentially expressed among
functionally distinct progenitors. HSCs were highly purified as CD150+ CD244− CD48− cells
while MPPs were CD244+ CD150− CD48− and most restricted progenitors were CD48+ …
Summary
To improve our ability to identify hematopoietic stem cells (HSCs) and their localization in vivo, we compared the gene expression profiles of highly purified HSCs and non-self-renewing multipotent hematopoietic progenitors (MPPs). Cell surface receptors of the SLAM family, including CD150, CD244, and CD48, were differentially expressed among functionally distinct progenitors. HSCs were highly purified as CD150+CD244CD48 cells while MPPs were CD244+CD150CD48 and most restricted progenitors were CD48+CD244+CD150. The primitiveness of hematopoietic progenitors could thus be predicted based on the combination of SLAM family members they expressed. This is the first family of receptors whose combinatorial expression precisely distinguishes stem and progenitor cells. The ability to purify HSCs based on a simple combination of SLAM receptors allowed us to identify HSCs in tissue sections. Many HSCs were associated with sinusoidal endothelium in spleen and bone marrow, though some HSCs were associated with endosteum. HSCs thus occupy multiple niches, including sinusoidal endothelium in diverse tissues.
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