Protective effect of a colchicine derivative in mice exposed to X-radiation
WW Smith - Science, 1958 - science.org
WW Smith
Science, 1958•science.orgMice Exposed to X-radiation Page 1 muscle (5) were chromatographed on a mixture of silicic
acid and Celite (6) (3: 1 by weight). The cardiolipin was adsorbed on the column from benzene,
and the column was developed with chloroform containing ethanol (5 percent by volume). The
column was extruded, and the zones were made visible by streaking with an alkaline
permanganate solution (7). The zones were cut and eluted with ethanol, and the eluate was
concentrated to dryness under reduced pressure at room temperature. Approximately 50 percent …
acid and Celite (6) (3: 1 by weight). The cardiolipin was adsorbed on the column from benzene,
and the column was developed with chloroform containing ethanol (5 percent by volume). The
column was extruded, and the zones were made visible by streaking with an alkaline
permanganate solution (7). The zones were cut and eluted with ethanol, and the eluate was
concentrated to dryness under reduced pressure at room temperature. Approximately 50 percent …
7. L. Zechmeister and W. H. McNeeley, J. Am. Chem. Soc. 64, 1919 (1942); B. W. Lew, M. L. Wolfrom, RM Goepp, Jr., ibid. 67, 1865 (1945); 68, 1449 (1946). 8. One column length istaken to bethe volume of developer necessary to just wet all the adsorbent.
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