Melanoma-reactive T cells in the bone marrow of melanoma patients: association with disease stage and disease duration
J Müller-Berghaus, K Ehlert, S Ugurel, V Umansky… - Cancer research, 2006 - AACR
J Müller-Berghaus, K Ehlert, S Ugurel, V Umansky, M Bucur, V Schirrmacher, P Beckhove…
Cancer research, 2006•AACRUsing IFN-γ enzyme-linked immunospot, we investigated reactivity of T cells from bone
marrow and peripheral blood to melanoma lysate-pulsed autologous dendritic cells in 40
melanoma patients. Melanoma-reactive T cells were present in the bone marrow of seven
patients and in peripheral blood of four patients. In the bone marrow, melanoma-reactive T
cells were present in 6 of 21 stage IV patients and in 1 of 10 stage III patients, whereas none
were detected in stage I to II patients (0 of 9). The occurrence of tumor-reactive bone marrow …
marrow and peripheral blood to melanoma lysate-pulsed autologous dendritic cells in 40
melanoma patients. Melanoma-reactive T cells were present in the bone marrow of seven
patients and in peripheral blood of four patients. In the bone marrow, melanoma-reactive T
cells were present in 6 of 21 stage IV patients and in 1 of 10 stage III patients, whereas none
were detected in stage I to II patients (0 of 9). The occurrence of tumor-reactive bone marrow …
Abstract
Using IFN-γ enzyme-linked immunospot, we investigated reactivity of T cells from bone marrow and peripheral blood to melanoma lysate-pulsed autologous dendritic cells in 40 melanoma patients. Melanoma-reactive T cells were present in the bone marrow of seven patients and in peripheral blood of four patients. In the bone marrow, melanoma-reactive T cells were present in 6 of 21 stage IV patients and in 1 of 10 stage III patients, whereas none were detected in stage I to II patients (0 of 9). The occurrence of tumor-reactive bone marrow T cells in melanoma patients was associated with advanced disease stage, disease duration and tumor load, and independent of treatment. These findings provide new insights into the generation of T-cell responses in melanoma patients. (Cancer Res 2006; 66(12): 5997-6001)
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