An Immuno-PF2D-MS/MS Proteomic Approach for Bacterial Antigenic Characterization: To Bacillus and Beyond
V Ruelle, N Falisse-Poirrier, B ElMoualij… - Journal of Proteome …, 2007 - ACS Publications
V Ruelle, N Falisse-Poirrier, B ElMoualij, D Zorzi, O Pierard, E Heinen, E De Pauw, W Zorzi
Journal of Proteome Research, 2007•ACS PublicationsWe are confronted daily to unknown microorganisms that have yet to be characterized,
detected, and/or analyzed. We propose, in this study, a multidimensional strategy using
polyclonal antibodies, consisting of a novel proteomic tool, the ProteomeLab PF2D, coupled
to immunological techniques and mass spectrometry (i-PF2D-MS/MS). To evaluate this
strategy, we have applied it to Bacillus subtilis, considered here as our unknown bacterial
model. Keywords: Bacillus subtilis• flagellin• in-gel digest• mass spectrometry• PF2D
detected, and/or analyzed. We propose, in this study, a multidimensional strategy using
polyclonal antibodies, consisting of a novel proteomic tool, the ProteomeLab PF2D, coupled
to immunological techniques and mass spectrometry (i-PF2D-MS/MS). To evaluate this
strategy, we have applied it to Bacillus subtilis, considered here as our unknown bacterial
model. Keywords: Bacillus subtilis• flagellin• in-gel digest• mass spectrometry• PF2D
We are confronted daily to unknown microorganisms that have yet to be characterized, detected, and/or analyzed. We propose, in this study, a multidimensional strategy using polyclonal antibodies, consisting of a novel proteomic tool, the ProteomeLab PF2D, coupled to immunological techniques and mass spectrometry (i-PF2D-MS/MS). To evaluate this strategy, we have applied it to Bacillus subtilis, considered here as our unknown bacterial model.
Keywords: Bacillus subtilis • flagellin • in-gel digest • mass spectrometry • PF2D
ACS Publications