[HTML][HTML] Construction of stable coxsackievirus and adenovirus receptor-expressing 3T3-L1 cells
DJ Orlicky, J DeGregori, J Schaack - Journal of lipid research, 2001 - ASBMB
Abstract 3T3-L1 cells have been used as a model to study the differentiation and physiology
of adipocytes. Exogenous expression of proteins in these cells offers the prospect of
understanding the protein's function (s) in adipose tissue. Viral vectors, in particular,
adenovirus, have proven to be a powerful means for introduction of genes into many cell
types. However, we have previously shown that 3T3-L1 cells are inefficiently transduced by
adenovirus (Orlicky, DJ, and J. Schaack. 2001. J. Lipid Res. 42: 460–466). To overcome the …
of adipocytes. Exogenous expression of proteins in these cells offers the prospect of
understanding the protein's function (s) in adipose tissue. Viral vectors, in particular,
adenovirus, have proven to be a powerful means for introduction of genes into many cell
types. However, we have previously shown that 3T3-L1 cells are inefficiently transduced by
adenovirus (Orlicky, DJ, and J. Schaack. 2001. J. Lipid Res. 42: 460–466). To overcome the …