The mechanisms underlying the ability of caloric restriction (CR) to extend life span and
enhance stress responsiveness remain elusive. Progress in this area has been slow due to
the complexities of using animals for CR studies and assessing life span as the measure of
CR effectiveness. It is therefore of great interest to develop in vitro models of CR. Here we
use sera obtained from either Fisher 344 rats or Rhesus monkeys that were fed ad libitum
(AL) or CR diets to culture various cell types. We show that treatment of cultured cells with …