Ascorbic acid and in situ kinetics: a new approach to vitamin requirements

M Levine, KR Dhariwal, PW Washko, JD Butler… - The American journal of …, 1991 - Elsevier
Ascorbic acid requirements are based on preventing the deficiency disease scurvy and on
urinary excretion of vitamin C. We proposed the first quantitative approach to determining
optimal requirements for ascorbic acid and other vitamins, called in situ kinetics. In situ
kinetics biochemically is based on the application of Michaelis-Menten reaction kinetics to
ascorbic acid-dependent reactions in situ. Clinically in situ kinetics is based on determining
vitamin availability to tissues so that cell-specific reactions can occur. The biochemical …