[HTML][HTML] Distinct Characteristics of the Basal Activities of Adenylyl Cyclases 2 and 6 (∗)

JP Pieroni, A Harry, J Chen, O Jacobowitz… - Journal of Biological …, 1995 - ASBMB
Regulation of basal activities of adenylyl cyclase (AC) 2 and 6, expressed in Sf9 cells by
infection with recombinant baculovirus, was studied. An antipeptide antibody that recognizes
AC2 and AC6 with equal sensitivity was used to establish that equivalent levels were
expressed. Basal activities of AC2 and AC6 were compared at varying concentrations of Mg
2+ or Mn 2+ ions; AC2 had 15-and 10-fold greater activity than AC6, respectively. At 20 mM
Mg 2+, the K m values for ATP were 88 and 39 μM for AC2 and AC6, respectively, whereas …