[HTML][HTML] Masking and unmasking maternal mRNA: The role of polyadenylation, transcription, splicing, and nuclear history
F Meric, AM Searfoss, M Wormington… - Journal of Biological …, 1996 - ASBMB
We establish that masked mRNAs synthesized from exogenous plasmid templates
microinjected into the nuclei of Xenopus oocytes are translationally activated (unmasked) on
oocyte maturation concomitant with polyadenylation. Synthetic mRNA injected into the
cytoplasm of the oocyte is translated over an order of magnitude more efficiently than is the
cognate mRNA synthesized in vivo. Both mRNA synthesized in vivo and mRNA
microinjected into the oocyte cytoplasm require a cytoplasmic polyadenylation element in …
microinjected into the nuclei of Xenopus oocytes are translationally activated (unmasked) on
oocyte maturation concomitant with polyadenylation. Synthetic mRNA injected into the
cytoplasm of the oocyte is translated over an order of magnitude more efficiently than is the
cognate mRNA synthesized in vivo. Both mRNA synthesized in vivo and mRNA
microinjected into the oocyte cytoplasm require a cytoplasmic polyadenylation element in …