[HTML][HTML] Characterization of NOBOX DNA binding specificity and its regulation of Gdf9 and Pou5f1 promoters
Y Choi, A Rajkovic - Journal of Biological Chemistry, 2006 - ASBMB
Nobox (newborn ovary homeobox gene) deficiency disrupts early folliculogenesis and the
expression of oocyte-specific genes in mice. Here, we identified several cis-acting sites,
TAATTG, TAGTTG, and TAATTA as NOBOX DNA binding elements (NBEs) using a library of
randomly generated oligonucleotides by cyclic amplification of sequence target assay and
mutation analyses. We show that NOBOX preferentially binds to the NOBOX binding
elements with high affinity. In addition, we found that promoter regions of mouse Pou5f1 and …
expression of oocyte-specific genes in mice. Here, we identified several cis-acting sites,
TAATTG, TAGTTG, and TAATTA as NOBOX DNA binding elements (NBEs) using a library of
randomly generated oligonucleotides by cyclic amplification of sequence target assay and
mutation analyses. We show that NOBOX preferentially binds to the NOBOX binding
elements with high affinity. In addition, we found that promoter regions of mouse Pou5f1 and …