Effects of Histone Deacetylase Inhibitor (HDACi); Trichostatin-A (TSA) on the expression of housekeeping genes
A Mogal, SA Abdulkadir - Molecular and cellular probes, 2006 - Elsevier
In quantitative RT-PCR (qRT-PCR), analysis of gene expression is dependent on
normalization using housekeeping genes such as 18S rRNA, GAPDH and β actin. However,
variability in their expression has been reported to be caused by factors like drug treatment,
pathological states and cell-cycle phase. An emerging area of cancer research focuses on
identifying the role of epigenetic alterations such as histone modifications and DNA
methylation in the initiation and progression of cancer. Histone acetylation is the best …
normalization using housekeeping genes such as 18S rRNA, GAPDH and β actin. However,
variability in their expression has been reported to be caused by factors like drug treatment,
pathological states and cell-cycle phase. An emerging area of cancer research focuses on
identifying the role of epigenetic alterations such as histone modifications and DNA
methylation in the initiation and progression of cancer. Histone acetylation is the best …