[HTML][HTML] Divergent roles of SHP-2 in ERK activation by leptin receptors
C Bjørbæk, RM Buchholz, SM Davis, SH Bates… - Journal of Biological …, 2001 - ASBMB
The protein tyrosine phosphatase SHP-2 has been proposed to serve as a regulator of leptin
signaling, but its specific roles are not fully examined. To directly investigate the role of SHP-
2, we employed dominant negative strategies in transfected cells. We show that a
catalytically inactive mutant of SHP-2 blocks leptin-stimulated ERK phosphorylation by the
long leptin receptor, ObRb. SHP-2, lacking two C-terminal tyrosine residues, partially inhibits
ERK phosphorylation. We find similar effects of the SHP-2 mutants after examining …
signaling, but its specific roles are not fully examined. To directly investigate the role of SHP-
2, we employed dominant negative strategies in transfected cells. We show that a
catalytically inactive mutant of SHP-2 blocks leptin-stimulated ERK phosphorylation by the
long leptin receptor, ObRb. SHP-2, lacking two C-terminal tyrosine residues, partially inhibits
ERK phosphorylation. We find similar effects of the SHP-2 mutants after examining …