[PDF][PDF] A simplified spectrophotometric determination of ester groups in lipids
G Thomas Passamanti - 1959 - deepblue.lib.umich.edu
G Thomas Passamanti
1959•deepblue.lib.umich.eduOur laboratory's need for an extremely simple yet highly sensitive and accurate assay of lipid
ester groupings in large numbers of ehromatogi-oDhic samples prompted the development
of the method reported here. It is based on a hydroxylaminolysis in which an ester reacts
with alkaline hydroxylamine to form a hydroxamie acid; the latter forms a purple iron-chelate
complex in the presence of acid ferric perchlorate. This method is a modification of more
lahorlous procedures ia and its range extends to 4. oo Fequiv. ester. A standard curve was …
ester groupings in large numbers of ehromatogi-oDhic samples prompted the development
of the method reported here. It is based on a hydroxylaminolysis in which an ester reacts
with alkaline hydroxylamine to form a hydroxamie acid; the latter forms a purple iron-chelate
complex in the presence of acid ferric perchlorate. This method is a modification of more
lahorlous procedures ia and its range extends to 4. oo Fequiv. ester. A standard curve was …
Our laboratory's need for an extremely simple yet highly sensitive and accurate assay of lipid ester groupings in large numbers of ehromatogi-oDhic samples prompted the development of the method reported here. It is based on a hydroxylaminolysis in which an ester reacts with alkaline hydroxylamine to form a hydroxamie acid; the latter forms a purple iron-chelate complex in the presence of acid ferric perchlorate. This method is a modification of more lahorlous procedures ia and its range extends to 4. oo Fequiv. ester.
A standard curve was determined on samples of tristearin, tripalmitin, methyl stearate, or methyl palmitate (California Foundation for Biochemical Research). The weighed lipid was dissolved in Fisher reagent-grade chloroform. Aliquots of the standards were pipetted into test tubes (85>~ 15 ram), and the solvent removed under infrared lamps. Acetone (about 0, 5 ml) was routinely added to all samples at this point to insure complete removM of the chloroform. The acetone was agai: x evaporated under infrared lamps.
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