[HTML][HTML] AMP-activated protein kinase is required for the lipid-lowering effect of metformin in insulin-resistant human HepG2 cells
The antidiabetic drug metformin stimulates AMP-activated protein kinase (AMPK) activity in
the liver and in skeletal muscle. To better understand the role of AMPK in the regulation of
hepatic lipids, we studied the effect of metformin on AMPK and its downstream effector,
acetyl-CoA carboxylase (ACC), as well as on lipid content in cultured human hepatoma
HepG2 cells. Metformin increased Thr-172 phosphorylation of the α subunit of AMPK in a
dose-and time-dependent manner. In parallel, phosphorylation of ACC at Ser-79 was …
the liver and in skeletal muscle. To better understand the role of AMPK in the regulation of
hepatic lipids, we studied the effect of metformin on AMPK and its downstream effector,
acetyl-CoA carboxylase (ACC), as well as on lipid content in cultured human hepatoma
HepG2 cells. Metformin increased Thr-172 phosphorylation of the α subunit of AMPK in a
dose-and time-dependent manner. In parallel, phosphorylation of ACC at Ser-79 was …