Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays

T Mosmann - Journal of immunological methods, 1983 - Elsevier
A tetrazolium salt has been used to develop a quantitative colorimetric assay for mammalian
cell survival and proliferation. The assay detects living, but not dead cells and the signal
generated is dependent on the degree of activation of the cells. This method can therefore
be used to measure cytotoxicity, proliferation or activation. The results can be read on a
multiwell scanning spectrophotometer (ELISA reader) and show a high degree of precision.
No washing steps are used in the assay. The main adavantages of the colorimetric assay …