[HTML][HTML] Tyrosine phosphorylation of the integrin β3 subunit regulates β3 cleavage by calpain
X Xi, P Flevaris, A Stojanovic, A Chishti… - Journal of Biological …, 2006 - ASBMB
Outside-insignalingofβ 3 integrinsinducesandrequiresphosphorylation at tyrosine 747 (Tyr
747) and tyrosine 759 (Tyr 759) of the β 3 subunit, but the mechanism for this requirement is
unclear. On the other hand, a key consequence of integrin signaling, cell spreading, is
inhibited by calpain cleavage of β 3 cytoplasmic domain. Here we show that β 3 tyrosine
phosphorylation inhibits calpain cleavage. Mutating both tyrosines to phenylalanine
sensitizes β 3 to calpain cleavage. Furthermore, phosphorylation at Tyr 747 and Tyr 759 of β …
747) and tyrosine 759 (Tyr 759) of the β 3 subunit, but the mechanism for this requirement is
unclear. On the other hand, a key consequence of integrin signaling, cell spreading, is
inhibited by calpain cleavage of β 3 cytoplasmic domain. Here we show that β 3 tyrosine
phosphorylation inhibits calpain cleavage. Mutating both tyrosines to phenylalanine
sensitizes β 3 to calpain cleavage. Furthermore, phosphorylation at Tyr 747 and Tyr 759 of β …