In-depth studies of innate immunity require efficient genetic manipulation of macrophages,
which is especially difficult in primary macrophages. We have developed a lentiviral system
for inducible gene expression both in macrophage cell lines and in primary macrophages. A
transgenic mouse strain C3H. TgN (SRA–rtTA) that expresses reverse tetracycline
transactivator (rtTA) under the control of macrophage-specific promoter, a modified human
Scavenger Receptor A (SRA) promoter was generated. For gene delivery, we constructed a …