[PDF][PDF] Conditional conversion of ES cells to skeletal muscle by an exogenous MyoD1 gene
I Dekel, Y Magal, S Pearson-White, CP Emerson… - New Biol, 1992 - researchgate.net
A variety of differentiated cell types can be converted to skeletal muscle cells following
transfection with the myogenic regulatory gene MyoD1. To determine whether multipotent
embryonic stem (ES) cells respond similarly, cultures of two ES cell lines were
electroporated with a MyoD1 cDNA driven by the (B-actin promoter. All transfected clones,
carrying a single copy of the exogenous gene, expressed high levels of MyoD1 mRNA.
Surprisingly, although maintained in mitogen-rich medium, this ectopic expression was …
transfection with the myogenic regulatory gene MyoD1. To determine whether multipotent
embryonic stem (ES) cells respond similarly, cultures of two ES cell lines were
electroporated with a MyoD1 cDNA driven by the (B-actin promoter. All transfected clones,
carrying a single copy of the exogenous gene, expressed high levels of MyoD1 mRNA.
Surprisingly, although maintained in mitogen-rich medium, this ectopic expression was …