Methods for myoblast transplantation

TA Rando, HM Blau - Methods in cell biology, 1997 - Elsevier
Publisher Summary This chapter focuses on the techniques of transplanting myoblasts
derived from primary cultures of mouse muscle. Myoblast transplantation provides a unique
means to effect in vivo gene transfer because of the ability of implanted myoblasts to fuse
with host myofibers. Among the limitations to more widespread use of myoblast
transplantation is the time required for growth, purification, and labeling of cells and for
detailed transplant analysis. For most applications of myoblast transplantation, it is essential …