[HTML][HTML] Phosducin-like protein regulates G-protein βγ folding by interaction with tailless complex polypeptide-1α: dephosphorylation or splicing of PhLP turns the …
J Humrich, C Bermel, M Bunemann, L Härmark… - Journal of Biological …, 2005 - ASBMB
Phosducin-like protein (PhLP) exists in two splice variants PhLP LONG (PhLP L) and PhLP
SHORT (PhLP S). Whereas PhLP L directly inhibits Gβγ-stimulated signaling, the G βγ-
inhibitory mechanism of PhLP S is not understood. We report here that inhibition of Gβγ
signaling in intact HEK cells by PhLP S was independent of direct Gβγ binding; however,
PhLP S caused down-regulation of Gβ and Gγ proteins. The down-regulation was partially
suppressed by lactacystine, indicating the involvement of proteasomal degradation. N …
SHORT (PhLP S). Whereas PhLP L directly inhibits Gβγ-stimulated signaling, the G βγ-
inhibitory mechanism of PhLP S is not understood. We report here that inhibition of Gβγ
signaling in intact HEK cells by PhLP S was independent of direct Gβγ binding; however,
PhLP S caused down-regulation of Gβ and Gγ proteins. The down-regulation was partially
suppressed by lactacystine, indicating the involvement of proteasomal degradation. N …