Efficiency, efficacy and adverse effects of adenovirus versus liposome-mediated ex vivo immunosuppressive cytokine gene transfer on the functional cardiac allografts

L Sen, Y Hong, H Luo, N Zhou, M Vargas… - The Journal of Heart …, 2001 - jhltonline.org
Background: Gene therapy may represent an effective strategy to modulate lung graft
ischemia-reperfusion injury. We investigated recipient intramuscular (IM) naked plasmid
gene transfer of TGF-1 as a means to ameliorate lung graft ischemia-reperfusion injury.
Material and Method: Preliminary studies in F344 rats, demonstrated that gastrocnemius
muscle (IM) transfection of TGF-1 active increased muscle and plasma protein expression
after 24hrs and 48hrs. Seven recipient groups (n8) received IM injection of naked plasmid …