[HTML][HTML] Low levels of expression of leptin receptor at the cell surface result from constitutive endocytosis and intracellular retention in the biosynthetic pathway
S Belouzard, D Delcroix, Y Rouille - Journal of Biological Chemistry, 2004 - ASBMB
The leptin receptor is mainly localized in intracellular compartments in target tissues. To
study the mechanisms leading to this intracellular localization, two main isoforms of leptin
receptors, OB-Ra and OB-Rb, were expressed in HeLa cells. Both isoforms were localized at
steady state in the trans-Golgi network, in endosomes, and to a lesser extent, at the cell
surface. They turned over with a half-life of less than 2 h. Both isoforms of leptin receptors
were constitutively endocytosed in a ligand-independent manner and degraded in …
study the mechanisms leading to this intracellular localization, two main isoforms of leptin
receptors, OB-Ra and OB-Rb, were expressed in HeLa cells. Both isoforms were localized at
steady state in the trans-Golgi network, in endosomes, and to a lesser extent, at the cell
surface. They turned over with a half-life of less than 2 h. Both isoforms of leptin receptors
were constitutively endocytosed in a ligand-independent manner and degraded in …